Day 1 :
- Applied Microbiology | Clinical Microbiology | Biotechnology
Session Introduction
El Hadji Seydou Mbaye
1International Agency for Research on Cancer (IARC/WHO), France
Title: Human Papillomavirus Infection in genital Women in four regions of Senegal
Biography:
Dr. El Hadji Seydou Mbaye was born in 1978 in Kaolack a region of Senegal. During 2008-2013, he earned his PhD in Biology and Human Pathologies with the collaboration of the International Agency for Research on Cancer (IARC) /WHO, Lyon (France); 2006-2007 : Master of Life and Health, Specialty Biology of microorganisms, Virology in Louis Pasteur University of Strasbourg (France); 2005-2006 : Master of Life and Health, option of Immuno-physiopathology in Louis Pasteur University of Strasbourg (France); 2004-2005 : License of Biochemistry in Louis Pasteur University of Strasbourg (France); 2002-2004 : General Degree in Sciences and Technologies in University of METZ (France).
Abstract:
INTRODUCTION:
Cervical cancer is the most frequent cancer among women in Senegal. However, there are few data concerning the HPV types inducing neoplasia and cervical cancers and their prevalence, in the general population of Senegal
AIMS:
The aim of this study is to determine the prevalence of HPV infection in Senegalese women aged from 18 years and older.
MATERIALS AND METHODS:
A study was performed on 498 cervix samples collected from healthy women aged 18 and older in Dakar. 438 other samples were collected from three other regions, Thiès, Saint Louis and Louga. The samples were screened for 21 HPV genotypes using an HPV type-specific E7 PCR bead-based multiplex genotyping assay (TS-MPG) which is a laboratory-developed method for the detection of HPV.
RESULTS:
The prevalence for pHR/HR-HPV in the region of Dakar was 20.68%. HPV 52 (3.21%) was the most prevalent HPV type, followed by HPV 16 (3.01%) and HPV 31 (3.01%). In the regions of Thiès, Louga and Saint Louis, the prevalence for pHR/HR-HPV was 29.19%, 23.15% and 20%, respectively
CONCLUSION:
The study revealed the specificity of the HR-HPV prevalence in Dakar and other regions of Senegal. The patterns differ from the one observed in the other regions of the world and rise the issue of the development of vaccination program in the country. Such a program should take into account the real HPV prevalence for an effective protection of HPV-associated diseases.
Xiaoge Sun
Animal Science and Technology, China Agricultural University, china
Title: Ruminal pH, VFA, microbiota, and performance of high-yield dairy cows supplemented with saccharomyces cerevisiae culture
Biography:
Xiaoge sun, Shu Zhang, Erdan Wang and Na Lu are PhD studies from College of Animal Science and Technology, China Agricultural University. Wei Wang is an assistant professor of China Agricultural University.
Abstract:
Dramatic increases in milk yields in recent decades have created challenges in terms of rumen pH and microbial health which ultimately impact on dairy cow health. The objective of this study was to assess effects on ruminal pH, Volatile Fatty Acid (VFA), microbiota, and performance of high-yield dairy cows by supplementing saccharomyces cerevisiae culture (SCC). Forty Holstein cows were divided into two groups based on their milk yield, days of milk, and parity, were fed the same basal ration diet that did or did not contain 100 g of SCC /cow per d. Individual dry matter intake (DMI) and milk yield were recorded each day. Rumen fluid and milk samples were collected after 2 hours of morning feeding at intervals of 15 days during the experiment period. The data showed that average rumen pH was increased by 0.19 (P=0.09) when SCC was supplemented than when no SCC was provided. Supplemented cow consumed 0.28 kg (P<0.05) extra DM/d during the trial period. Those supplemented with SCC produced 1.36 kg (P<0.05) more milk/cow per d than did non-supplemented cows. Milk fat percentage was higher (4.11 vs. 3.96%) for cows receiving SCC. There were no differences in milk protein percentage. Rumen fluid VFA concentration was not statistically affected by SCC, but was numerically higher acetic and lower propionic for supplemented cows. SCC-supplemented cows had a greater relative abundance of prevotellaceae, Succinivibrionaceae, Burkholderiaceae, Fibrobacteraceae, Lactobacillaceae, and lower relative abundance of Spirochaetaceae, Methanobacteriaceae, Enterobacteriaceae than the unsupplemented cows. It had greater functions on xylanolysis, fermentation, cellulolysis in rumen in terms of the KEGG function prediction analysis. This study demonstrated that high-yield lactation cows receiving supplemental SCC produced more milk and consumed more DM. Cows consuming SCC potentially enhanced rumen good bacteria and depressed maleficent bacteria.
Omasan Eyinmisan Urhie
Northeast Agricultural University, china
Title: Lysine 265 is critical for NhaD-type Na+/H+ antiporter from Halomonas hydrothermalis
Biography:
Dr Mrs Omasan Eyinmisan Urhie had completed her PhD at the age of 33 from Northeast Agricultural University, Harbin China and she is currently running an MSc in Biomedical Science (Medical Microbiology option) in the University of the West of England, Bristol United Kingdom. She has published 4 papers in reputable journals and Lectured in Edwin Clark University, Kiagbodo Delta State Nigeria.
Abstract:
The Nh-NhaD is an Na+/H+ antiporter from Halomonas hydrothermalis belonging to a new family of bacterial Na+/H+ antiporters of the NhaD family. In this present work, two amino acids Lys 265 and Asp 167 were substituted with alanine so as to identify amino acids vital for the antiporter activity. The deduced amino acid sequence of Hh-NhaD comprises 492 residues with calculated molecular weight of 54367.39Da and a PI of 8.82. KNabc/pUC-om-NhaD, KNabc/pUC 18, Knabc/pEasyT3 vector (negative controls), Knabc/pEasyT3-NhaD-His-tag, Knabc/pEasyT3-NhaDN167 and KNabc/pEasyT3-NhaDK265 were grown in LBK medium containing 0-0.8mol/L NaCl or 0-300mM LiCl in order to test the ability of Hh-NhaD to confer salt tolerance. KNabc/pUC-om-NhaD, Knabc/pEasy T3-NhaD-His-tag and Knabc/pEasyT3-NhaDN167 were able to grow in the presence of upto 0.6mol/L NaCl while KNabc/pEasyT3-NhaDK265 could not survive in the presence of LBK medium containing 0.2mol/L NaCl. The same trend was observed for LiCl test. The expression of Hh_nhaD conferred on E. coli KNabc cells the ability to grow under alkaline conditions in KNabc/pUC–om-NhaD and Knabc/pEasyT3-NhaD-His-tag. KNabc/pEasyT3-NhaDK265 could not survive under alkaline condition of pH 8.0 which indicates the importance of Lys 265 residue in pH regulation. The function of Hh_NhaD was identified in detail and the importance of Lys 265 in pH regulation and cation transport was proven in this study. Hh_NhaD a membrane protein was experimentally established to exhibit Na+(Li+)/H+ antiport activity with pH dependency. Hh_nhaD gene encode a novel NhaD-type Na+/H+ antiporter and Lys 265 is very essential for cation binding and pH regulation in NhaD from H. hydrothermalis.
KEYWORDS: Halomonas hydrothermalis, moderately haloalkaliphilic bacterium, Na+(Li+)/H+ antiporter, NhaD, pH dependent activity profile, site directed mutagenesis and Lys K265.
Natia Tamarashvili
t G. Eliava Institute of Bacteriophages, Microbiology and Virology, Georgia
Title: The Use of Bacteriophages for Decontamination of Experimentally Contaminated Chicken Breast Meat
Biography:
Xiaoge sun, Shu Zhang, Erdan Wang and Na Lu are PhD studies from College of Animal Science and Technology, China Agricultural University. Wei Wang is an assistant professor of China Agricultural University.
Abstract:
wang@cau.edu.cn Dramatic increases in milk yields in recent decades have created challenges in terms of rumen pH and microbial health which ultimately impact on dairy cow health. The objective of this study was to assess effects on ruminal pH, Volatile Fatty Acid (VFA), microbiota, and performance of high-yield dairy cows by supplementing saccharomyces cerevisiae culture (SCC). Forty Holstein cows were divided into two groups based on their milk yield, days of milk, and parity, were fed the same basal ration diet that did or did not contain 100 g of SCC /cow per d. Individual dry matter intake (DMI) and milk yield were recorded each day. Rumen fluid and milk samples were collected after 2 hours of morning feeding at intervals of 15 days during the experiment period. The data showed that average rumen pH was increased by 0.19 (P=0.09) when SCC was supplemented than when no SCC was provided. Supplemented cow consumed 0.28 kg (P<0.05) extra DM/d during the trial period. Those supplemented with SCC produced 1.36 kg (P<0.05) more milk/cow per d than did non-supplemented cows. Milk fat percentage was higher (4.11 vs. 3.96%) for cows receiving SCC. There were no differences in milk protein percentage. Rumen fluid VFA concentration was not statistically affected by SCC, but was numerically higher acetic and lower propionic for supplemented cows. SCC-supplemented cows had a greater relative abundance of prevotellaceae, Succinivibrionaceae, Burkholderiaceae, Fibrobacteraceae, Lactobacillaceae, and lower relative abundance of Spirochaetaceae, Methanobacteriaceae, Enterobacteriaceae than the unsupplemented cows. It had greater functions on xylanolysis, fermentation, cellulolysis in rumen in terms of the KEGG function prediction analysis. This study demonstrated that high-yield lactation cows receiving supplemental SCC produced more milk and consumed more DM. Cows consuming SCC potentially enhanced rumen good bacteria and depressed maleficent bacteria. Key words: rumen, pH, VFA, microbia